BECAUSE THE INCIDENCE OF SKIN WOUNDS REQUIRING CLINICAL TREATMENT REPRESENTS A PUBLIC HEALTH PROBLEM WORLDWIDE, THE PRESENT WORK AIMS TO DEVELOP A BILAYER SCAFFOLD OF POLY (LACTIDE- CO- GLYCOLIDE) (PLGA)/FIBRIN ELECTROSPUN MEMBRANE AND FIBRIN HYDROGEL LAYER TO BE TESTED IN VIVO AS SKIN SUBSTITUTES. PRIMARY CULTURES OF FIBROBLASTS AND KERATINOCYTES WERE ISOLATED FROM ISOGENIC WISTAR KYOTO RATS (WKY). FIBROBLASTS WERE CULTIVATED IN THE FIBRIN HYDROGEL AND KERATINOCYTES ON THE ELECTROSPUN LAYER TO GENERATE A SKIN SUBSTITUTE USING AN AIR/LIQUID SYSTEM. SCAFFOLDS WERE TESTED IN A FULL-THICKNESS WOUND MODEL IN WKY RATS OF 3 MONTHS. THREE GROUPS WERE ANALYZED MACROSCOPICALLY AND MICROSCOPICALLY: 1 (BILAYER SCAFFOLD WITHOUT CELLS), 2 (HETEROTYPIC SKIN SUBSTITUTES), 3 (NEGATIVE CONTROL). PARTIAL RESULTS SHOWED A SCAB FORMATION AT DAY 14 IN ALL ANIMALS FROM GROUPS 1, 2, AND 3. NO SIGNS OF WOUND INFECTION WERE PRESENTED. ON DAY 14, ALL WOUNDS WERE RE-EPITHELIALIZED AND GRANULATION TISSUE WAS THICKER IN GROUP 2. IT COULD BE CONCLUDED THAT THE BILAYER SCAFFOLD IS THUS A PROMISING MATRIX TO BE USED AS A SKIN SUBSTITUTE. HOWEVER, IT WILL BE NECESSARY TO COMPLETE THE SAMPLE SIZE FOR EACH GROUP AND REALIZE HISTOLOGICAL AND IMMUNOENZYMATIC ASSAYS TO BETTER UNDERSTAND THE TISSUE REGENERATION PROCESS.