INTRODUCTION: SPINAL CORD INJURY (SCI) IS A SERIOUS DISEASE YET THERE IS NO TRULY SUCCESSFUL TREATMENT. IN THIS WORK, DIFFERENT OILS HAVE BEEN USED IN THE PREPARATION OF NANOCAPSULES (NCS), TO ENCAPSULATE THE NICOTINE FOR THE TREATMENT OF SCI AND COMPARE IT IN TERMS OF DIAMETER, POLYDISPERSITY INDEX (PDI), ZETA POTENTIAL, AND ENCAPSULATION EFFICIENCY (EE). METHODOLOGY: NCS WERE PREPARED USING POLY (LACTIC-CO-GLYCOLIC ACID) (PLGA) THROUGH THE TECHNIQUE OF INTERFACIAL POLYMER DEPOSITION. IN GROUP I, GRAPE SEED OIL WAS USED, IN GROUP II CASTOR OIL, IN GROUP III COPAIBA OIL, AND IN GROUP IV A?A? OIL. THE CHARACTERIZATION OF THE NCS WAS CONDUCTED IN ZETASIZER AND THE EE BY MASS SPECTROMETRY. RESULTS: THE NCS OF GROUP I EXHIBITED AN AVERAGE DIAMETER OF 214.9 NM, PDI OF 0.351, ZETA POTENTIAL OF -47.5 MV, AND EE OF 15.88%. GROUP II PRESENTED THE AVERAGE DIAMETER OF 207.7 NM, PDI OF 0.271, AND ZETA POTENTIAL OF -27.2 MV, AND EE OF 91.27%. GROUP III EXHIBITED AN AVERAGE DIAMETER OF 190.2 NM, PDI OF 0.421, ZETA POTENTIAL OF -35.5 MV, AND EE OF 37.3%. GROUP IV PRESENTED THE AVERAGE DIAMETER OF 121.4 NM, PDI OF 0.418, ZETA POTENTIAL OF -22.3 MV, AND EE OF 32.45%. CONCLUSIONS: THESE RESULTS REVEAL THAT ALL GROUPS HAVE ADEQUATE SIZE, ZETA POTENTIAL, AND POLYDISPERSION, WHICH ATTESTS TO THEIR STABILITY, BUT GROUP II HAS EXCELLENT EE COMPARED TO THE OTHER GROUPS. THE NEXT PHASE WILL BE THE EVALUATION OF THE NC RELEASE PROFILE.